A transient calcium-dependent chloride current in the immature Xenopus oocyte.
نویسندگان
چکیده
منابع مشابه
Calcium dependent variance of chloride current fluctuations in Xenopus laevis oocytes.
Calcium induced chloride currents were studied in Xenopus oocytes using the two-electrode voltage clamp technique. Fluctuations of chloride currents measured under voltage clamp were elicited by acetylcholine. Fluctuations were superimposed on a larger chloride current. These chloride currents are due to an increase of intracellular calcium. However, an injection of calcium into the cytoplasm e...
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Ca(2+) is the universal signal for egg activation at fertilization in all sexually reproducing species. The Ca(2+) signal at fertilization is necessary for egg activation and exhibits specialized spatial and temporal dynamics. Eggs acquire the ability to produce the fertilization-specific Ca(2+) signal during oocyte maturation. However, the mechanisms regulating Ca(2+) signaling differentiation...
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Xenopus oocytes are frequently utilized for in vivo expression of cellular proteins, especially ion channel proteins. A thorough understanding of the endogenous conductances and their regulation is paramount for proper characterization of expressed channel proteins. Here we detail a novel chloride current (ICl.swell) responsive to hypotonicity in Xenopus oocytes using the two-electrode voltage ...
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Objective(s): Failed fertilization after intra-cytoplasmic sperm injection (ICSI) is mainly attributed to failed oocyte activation and can be overcome by artificial oocyte activation (AOA). The present study aims to compare in vitro outcomes of ICSI following two different assisted oocyte activation chemical procedures (SrCl2 and Ionomycin) in sibling oocytes of ICSI candidates.Materials and Me...
متن کاملAnalysis of calcium activated chloride current fluctuations in Xenopus laevis oocytes.
Fluctuations of calcium activated chloride currents were investigated in oocytes of Xenopus laevis. The method of noise analysis and the model of chloride channels activation by calcium ions were used to estimate the chloride channels lifetime and the average frequency of current fluctuations, which depends on changes of cytoplasmic calcium concentration. This current fluctuations can be evoked...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1983
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1983.sp014852